Exact(4)
First, we visually compared alignments for congruence in their ability to align sections of the alignments (especially the inner core) using AltAVisT [54] and the overall sequence identity calculated from each alignment.
We compared alignments generated by several sequence and structure alignment programs to determine whether existing methods would align residues at a highly conserved position within RIPs (Lebeda and Olson, 1999).
Given the high evolutionary distance among the species compared, alignments with an e-value < 1e-03 were considered significant and a maximum of 20 hits were taken into account for each query.
To investigate a possible correlation between the presence of an L-8N motif and the conservation of the residues at positions -3, -2 and -1 relative to Asn-29, we compared alignments of L-8N repeats and ARs lacking an L-8N motif.
Similar(56)
One of the two compared alignment-free methods was developed by Li et al. in 2011 [ 18], the other was developed by Yu and Huang in 2013 [ 14].
The first was the development of the primer pair ENDOITSf and ENDOITSr (Table 1), created by comparing alignments of Atriplex ITS sequences against the ITS sequences of fungal isolates, and identifying potential fungal primers with minimal affinity for Atriplex DNA.
The invariant tryptophan starting the WRKYGQK motif was used as the reference point for comparing alignments.
In the absence of an objective universal score to compare alignments of different datasets, we chose the 15 largest alignments and visually inspected them for problems.
Consensus ITS-2 sequences, here called genotypes, were constructed manually by comparing alignments obtained using the Clustal W (version 1.83) algorithm [ 21].
This software compares alignments of the sample protein to other proteins with known structural information to determine a probable structure using statistics.
Using these simulated data, we explore the contribution of a variety of factors by comparing alignments that differ with respect to number of sequences, tree topology, alignment length, and gap pattern.
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