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We used four comparator datasets: (i) a set of 2699 Sanger dideoxy sequenced ESTs from L. sigmodontis (Mark Blaxter, unpublished), assembled into 1602 clusters spanning 0.9 Mb (see http://www.nematodes.org/nembase4/species_info.php?species=LSC).
Important methodological issues, including the need for additional comparator datasets, the potential bias from missing data, and the impact of the "no improvement" rule, will need to be addressed and long term follow-up of all patients is essential to secure meaningful results.
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They acknowledge that their sample of job ads is not representative of the overall population of jobs in the Chinese labour market, using the 2005 census in China as a comparator dataset.
At each footprint locus the data from the comparator dataset is added and the Wellington footprint score for the pooled data evaluated.
The Ontario comparator dataset might not be appropriate for other reasons, such as the natural course of the disease changing over time.
Estimating cost effectiveness from an observational cohort study with a historical comparator dataset has inherent problems that would be avoided in a randomised controlled trial.
Wellington-bootstrap then assesses if the change in footprint score is a consequence of the increase in read numbers after pooling reads or if the data from the comparator dataset makes a contribution to the footprint structure.
Results In the primary per protocol analysis, progression in disability was worse than that predicted and worse than that in the untreated comparator dataset ("deviation score" of 113%; excess in mean disability status scale 0.28).
The natural history BCMS comparator dataset comprised of 898 patient profiles with 7335 EDSS scores providing 6357 transitions between consecutive EDSS states, that is, 6357 'events' where EDSS values were recorded at consecutive visits.
Datasets from cultured human and mouse cells were also selected as comparators since the datasets should not be directly affected by plant sRNAs resulting from dietary exposure.
Of course, larger datasets and agreement on comparator groups are needed to ascertain any differences in genotype between different types of cohorts, whether those considered to be progressing along a continuum, or those considered as having dichotomous disease processes.
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