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The assessment of expression comparing different targets was determined by the ddCt comparative threshold (ΔΔCt) method.
comparative threshold.
The relative abundance of transcripts was calculated by the comparative threshold cycle (CT) method.
Estimates for gene expression were based on the comparative threshold cycle (Ct) method.
Expression profile in each specimen was assessed by using the comparative threshold cycle (2-ddCt) method.
Relative gene expression was calculated using the comparative threshold (Ct) method (2−ΔΔCt) [34].
To quantify the results we used the comparative threshold cycle method ΔΔC t).
The relative quantification of gene expression changes were performed using the comparative threshold (CT) method (ΔΔCT).
The data were analyzed using comparative threshold cycle method using GAPDH as internal control.
The relative amounts of specifically amplified cDNAs were calculated with the comparative threshold cycle (ΔΔCT) method.
The comparative threshold cycle (CT) method was used to calculate the relative concentrations.
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