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Comparative screening of gene expression libraries employing the potent industrial host Pichia pastoris for improving recombinant eukaryotic enzymes by protein engineering was an unsolved task.
The application of the proposed methodology is demonstrated for both flood fragility/risk assessment and comparative screening of various viable flood mitigation strategies for a non-engineered building made of cement bricks in Dar Es Salaam, Africa.
The comparative screening of miRNA content of MVs and adult stem cells may provide information on the mechanism of RNA accumulation within MVs.
One of these antigens is the cell-traversal protein for ookinetes and sporozoites (CelTOS) identified independently by two laboratories through either comparative screening of genomic databases for immunogenic antigens [6] or using suppression subtractive hybridization (SSH) of Plasmodium yoelii sporozoites versus merozoites [7].
The Western blots shown in Figure 1F were developed as individual strips in separate plastic bags at a dilution of 1 in 200 which results in a better definition of the bands but is not suited for comparative screening of large numbers of sera.
The comparative screening of the gene content was performed in RAST, IMG-ER and EDGAR [ 26].
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In one such effort, Mckerrow and co-workers carried out a comparative screen on a 909-member library of bioactive compounds against Leishmania donovani.
Our large-scale comparative screening demonstrated that most closely related bacteria tend to have the same isoforms of dnaE polymerases.
This article reports for the first time the comparative proteomic screening of several S100 protein members among a large group of breast cancer patients.
Case-control mutation screening, or its quantitative trait homolog of comparative mutation screening of individuals from the opposite ends of a trait spectrum, is emerging as a useful strategy for identifying and characterizing intermediate-risk susceptibility genes [ 6- 8, 10, 27- 29].
Given that Neurospora crassa has a close phylogenetic relationship with T. reesei and possesses a nearly complete set of genome-wide gene deletion mutants, thereby making it a powerful tool for use in genetic studies [ 21], we reasoned that comparative genomic screening of N. crassa mutants could be applied as an alternative approach to study functions of mutated genes in T. reesei RUTC-30.
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