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Quality of RNA was confirmed by gel electrophoresis and comparative quantities by RT-PCR with oligodT and GAPDH specific primers.
ADNI Aβln Aβ(1 40) and Aβln Aβ(1 42) values are placed underneath their comparative quantities.
The related gene expression was calculated using the comparative method for the relative quantity normalized to GAPDH gene expression.
MicroRNA expression levels (relative quantity, RQ) were calculated using the comparative Ct method as previously described and fold change (FC) was computed as (Livak and Schmittgen, 2001).
The comparative ΔΔCT method was employed to evaluate the relative quantity of each amplified product in the samples.
The relative quantity of target genes was calculated using the comparative threshold method (ΔΔCt) and using Ppib as endogenous control as previously described[55].
The relative quantity of miRNA expression was calculated using the comparative cycle threshold (ΔΔCt) method [ 27].
The comparative ΔΔCt method was used to calculate relative gene expression and expressed as a Log10 Relative Quantity [25].
The relative quantity of each RNA transcript was calculated with the comparative Ct (cycling threshold) method using the formula 2ΔCt.
Relative quantity of target cDNA was computed by using the comparative threshold (Ct) method.
The relative quantity of the RNA expression was calculated using the comparative CT method with the 7500 System SDS software [ 10].
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