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The observation that the PI gene is associated with analogous traits in humans demonstrates the effectiveness of the positional comparative candidate gene analysis that utilizes information about genes present in chromosomal regions with conserved synteny in other species.
Positional, comparative candidate gene analysis and previous quantitative trait loci linkage mapping results were used to search for candidate genes affecting milk production and reproduction traits in dairy cattle.
(1992b), following discovery that SCN4A, a strong comparative candidate gene, co-segregates with the disorder (Rudolph et al. 1992a).
Within this genomic segment, 4 of the 18 known EB comparative candidate genes were located in the bovine genome including the ITGB4 gene which contains the above mentioned large genomic deletion.
First, a recent dominant de novo mutation was hypothesised and the entire genome of the affected animal was therefore sequenced in order to detect all the variants in the known EB comparative candidate genes.
A total of 890 deletions were private deletions occurring only in the genome of the affected Charolais animal in which a single 4.8 kb deletion was detected in the region of one of the comparative candidate genes (ITGB4), starting at position 56,488,275 on cattle chromosome 19 (UMD3.1/bosTau6 assembly).
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A comparative positional candidate gene approach led to identification of the candidate gene coding acylcoA: diacylglycerol – acyltransferase 1 (DGAT1).
Genic SSR markers could represent the new class of functional markers, finding use in evolutionary studies, comparative mapping, candidate gene association mapping, gene discovery and molecular breeding (Shi et al. [2014]).
We herein considered UGDH as the most plausible comparative functional candidate gene affecting milk production traits.
Exactly the same comparative positional candidate gene strategy was used by Klungland et al.
Out of them, we herein focused on UGDH as the most plausible comparative functional candidate gene affecting milk production traits.
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