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The Mauve progressive multiple genome aligner was used to provide comparative alignment of genome features from L. erinacea, A. radiata and O. kenojei (16, 17).
Comparative alignment of these assemblies revealed 99.5% nucleotide identity (representing 3,599,169 alignable bases) among all three strains (Additional file 3).
The comparative alignment between VACV G8R, human and yeast PCNA protein sequences was created using T-Coffee with manual manipulations to ensure that the secondary structure that was being mapped contained no gaps.
As shown in Figure 1B, comparative alignment of the HKD motif amino acid sequence, which is essential for PLD enzymatic activity, demonstrated the presence of several differences between TM-PLDs and the PXPH-PLDs (see Discussion).
Using as a basis the number of annotated repeat insertions in the 12 species genomes (Caspi, unpublished) obtained through a comparative alignment method [30], we estimated the total number of TE insertions per Mb in the sequenced genomes.
A comparative alignment of the MNV signal with other known or suspected termination-reinitiation signals (Figure 8) reveals that Motif 1 is always present and that the stop and start codons of the termination-reinitiation site are in close proximity to each other.
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Comparative alignments between regions shared between all three holarctica genomes revealed 1364 polymorphic sites.
cDNA from unaffected dog skin obtained by RT-PCR was sequenced to verify the exon boundaries inferred from comparative alignments with the human and mouse orthologs.
We first determined 'optimal' sets of comparative alignments by maximizing the number of known ncRNAs, serving as positive controls, recovered by different prediction conditions (Text S1).
This hypothesis is also supported by our analysis of E protein comparative alignments between FE, SIB and WE subtypes that revealed more hydrophobic "ricinus" amino acid patterns, compared with "persulcatus", in correspondence with tick preference of WE or FE/SIB strains respectively.
Comparative alignments were visualized using MUMMER software [ 35] or the LAGAN algorithm [ 50].
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