Strikingly, 5320 out of 5727 experimentally verified and tested gene pairs are similarly expressed across both species showing comparable transcript abundances.
In total, 17 regulatory motifs were predicted for FA synthesis and elongation (92 genes), lipid degradation (74 genes), lipid desaturation (12 genes) and triacylglycerol (16 genes) processes considering gene clusters with comparable patterns in transcript abundance (Table 1; Figure 5).
Transcript abundance was comparable between 3-T10 and 4-T10, between 3-T50 and 4-T50 and between 3-T100 and 4-T100, although the T50 constructs exhibited abundance nearly twice as much as the others for reasons unknown.
These data are now directly comparable to previously published virtually-multiplexed transcript abundance data from this laboratory [15], or to Virtually-multiplexed transcript abundance data collected by others who use the NCI-funded (R24 CA 95806) Standardized Expression Measurement (SEM Centerr.
Induction of NRAMP1 upon Mn deficiency was moderate though and not comparable with the dramatic increase in IRT1 transcript abundance upon Fe deficiency.
The mean abundance of MIP-1β was slightly but not significantly higher in HIV-1-infected (median ΔCT 5.70), when compared with that of HIV-1-uninfected persons (median ΔCT 7.02, p = 0.225), whereas MIP-1α transcript abundance in BAL was comparable in HIV-1-infected and uninfected persons.
Both techniques have been previously applied to modelling transcript abundance as a function of transcriptional regulatory elements and demonstrated to yield comparable predictive performance [ 15– 15].
Even though currently RNA-seq is the most comprehensive methodological approach to assess transcript abundance and complexity, DGE is conceptually more comparable to microarrays.
Transcript abundance estimates from the two studies were directly comparable (data not shown).
To test this possibility, we first analyzed Tex19.1 transcript abundance in Ubr2−/− and wild type testes and found comparable levels (Fig. 5A).
Results of the QRT PCR of this study indicate that at the mRNA level in osseous, cartilaginous and muscular tissues of the spine transcript abundance of both VDR isoforms in female and male population is comparable.
