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For assessment of the degree of conservation between the Oct4-associated proteins and their orthologs, sequence identities of all mouse-to-human ortholog pairs of comparable sequence length in ENSEMBL release 57 were compared via a Mann-Whitney U test.
According to SPR studies, f-IPI (3a) bound more strongly (Keq = 2.4 × 108 M−1) and with comparable sequence selectivity to its cognate sequence 5′-ACGCGT-3′ when compared to its monoamino analog f-IPI (1).
A well-resolved separation profile was achieved and comparable sequence coverage was obtained by the CE-MS method (73%) compared to a representative UPLC-MS method (77%).
(This is an actual sequence, from chromosome 10; the comparable sequence in an elephant is ACCTCCCCTAATGTCA).
Data pre-processing is a process of transferring the original sequence to a comparable sequence.
The obtained library diversity explores a comparable sequence space as the donor-derived natural repertoire and, at the same time, is able to access novel recombined diversity due to lack of segmental linkage.
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Note that the described control sample-based confidence correction is based on the assumption that the compared datasets (IP and control) present comparable sequencing depth levels.
An alternative high-throughput sequencing approach, semiconductor-based sequencing using the Ion Torrent platform (Life Technologies, Carlsbad, CA) offers comparable sequencing data at quicker run speeds and lower cost relative to optical-based sequencers.
When the team injected embryos with comparable sequences from chimps and macaques, the gene activity was confined to the base of the limb.
In addition, both age groups demonstrated comparable sequence-specific learning as indicated by an increase in response times when switching from sequential to random finger movements [5], [6].
Within this set of amphioxus and vertebrate Hox-proteins, the most comparable sequences between the model organisms are amphioxus Hox13 and vertebrate Hox11, although the sequences should not be expected to provide functionally equivalent proteins.
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