On the other hand, all the truncated HE mutants14 with or without additional amino acid changes showed comparable knock-in efficiency to mock-transfected control, although Charpentier et al. showed that Cas9 fused with HE mutant (Cas9-HE) resulted in higher HDR efficiency compared to that of Cas914.
IPSC-Mac also phagocytosed GFP-labeled P. aeruginosa (PAO1) at 37 °C in comparable efficiency to PBMC-Mac, whereas no phagocytosis was observed at 4 °C, confirming active phagocytosis (Fig. 3b and Supplementary Figure 3a).
The compared gene assays were comparable in efficiency and over 96% for cDNA from samples and controls.
In treating synthetic dye wastewater, the extracted coagulant showed comparable treatment efficiency to the commercial coagulant.
Indeed, an mTOR kinase assay revealed that mTORC1 phosphorylates highly purified TFEB in vitro with comparable efficiency to other known mTORC1 substrates, and this phosphorylation dropped dramatically when mTORC1 was incubated with the S142A mutant version of TFEB.
The position of the lesion with respect to the histone core is also vitally important [26], [30], [35] as lesions facing "out", away from the histone, are processed with almost comparable efficiency to lesions contained in free DNA but lesions inward-facing and mid-way (meaning half-way between the histone core and solution) lesions are generally excised with much lower efficiency.
Embryos developed in this modified system with comparable efficiencies to our earlier study (Morris et al., 2012a), but their development was more easily recorded by confocal microscopy, as they were grown on optical-grade plastic.
Mr. Kaehny said there were numerous factors contributing to a slip in compliance, among them that many agencies had made it possible to file requests online, leading to an enormous increase in requests, without making any comparable advances in efficiency in their method of responding.
While other array reconfiguration approaches can offer comparable improvements in efficiency, the reduced complexity of the TDAR approach makes it the only scalable approach that can be practically applied to array reconfiguration in portable systems.
We found pure methanol extraction to be slightly better as compared to the two phase methanol/chloroform/water extraction in terms of introducing less degradation of the extracted metabolites while being comparable in extraction efficiency and repeatability.
