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The effect of Ro3303544 on the compaction of inflammatory cells after SCI was then investigated.
What is the mechanism whereby the accelerated compaction of inflammatory cells improves functional recovery?
To examine the possibility that the increased compaction of inflammatory cells upon Ro3303544 administration in vivo resulted from enhanced proliferation of reactive astrocytes, BrdU incorporation experiments were performed.
To focus on the compaction of inflammatory cells by reactive astrocytes, the protocol consisted of intraperitoneal administration of Ro3303544 for only the first 5 days after thoracic contusive SCI in mice (Fig 3A).
An association between compaction of inflammatory cells by migrating reactive astrocytes and a reduction in delayed neuronal damage, such as demyelination, has been previously reported (Herrmann et al, 2008; Okada et al, 2006).
Moreover, the observed increased compaction of inflammatory cells in vivo after Ro3303544 administration (Fig 4) suggests that the migration of astrocytes observed in the single-cell transwell assay more closely resembles the situation of in vivo reactive astrocytes and reveals that the in vitro wound scratch assay is not always relevant to in vivo CNS injury.
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Quantitative analysis revealed no significant difference in the number of BrdU-labelled reactive astrocytes surrounding the lesion, implying that the increased compaction of infiltrated inflammatory cells by Ro3303544 results from the migration of reactive astrocytes rather than from astrocyte proliferation (Fig 4D).
It's used to test the compaction of recently compacted roads.
Passionate debates of inflammatory topics.
The compaction of the soil means less absorption of rainfall.
Heavy steel-wheeled compactors may also be employed to achieve high-density compaction of the refuse.
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