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Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization.
At the end of UASB operation, microbial community characterization revealed satisfactory composition of microbes whereas morphology depicted rod-shaped archaea.
This work first reviews recent literature on microbial community characterization in indoor environments (primarily those that utilized molecular methods), paying particular attention to the level of assessment of influential built environment characteristics and the specific methods and procedures that were used to collect those data.
Previous MFC microbial ecology studies have used Sanger-based 16S rRNA sequencing for microbial community characterization.
Metagenomics is a powerful tool for viral and microbial community characterization since nucleic acids are isolated directly from environmental samples and sequenced, requiring no culturing, cloning, or a priori knowledge of what viruses may be present.
Using this more detailed microbial community characterization, it may be possible to move away from generic, single indicators to a community-indicator approach for assessing fecal contamination or environments conducive to pathogen growth.
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Compared to culture-based techniques, DNA sequencing of the bacterial 16S ribosomal RNA (rRNA) gene or whole metagenome shotgun (WMS) sequencing provides more precise microbial community characterizations.
Therefore, only these two types were considered in the final community characterizations (referred to in this manuscript as 'clade C' and 'clade D'clade
Importantly, our sampling effort does not appear to significantly influence measures of species diversity and dissimilarity in either the seed bank or the aboveground community characterizations.
Future studies on fungal community characterizations and bioprospection are recommended for these particular environments (including more thorough sampling efforts), because there is a high probability of very interesting findings.
Community-scale analyses can be readily refined in response to evolving knowledge of influenza biology and epidemiology, individual and community behavior, characterization of social contact networks, and mitigation options.
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