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To characterize the microbial communities, samples of microbial mats were collected using a 70% (v/v) ethanol-rinsed spatula, placed into sterile 15 ml plastic vials, and fixed in natural water using 2% (v/v) glutaraldehyde.
From within these communities, samples were balanced across age and sex, as described in detail elsewhere (25).
Because initial sampling was focused on endoparasite communities, samples from 2004 2005 consisted of complete gastrointestinal tracts frozen in liquid nitrogen.
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Leptospirillum group III was ubiquitous in the communities sampled in this study.
In contrast, in small communities sampling community-wide interactions is more precise.
Combined richness for all bacterial midgut communities sampled was 317 OTUs.
They had provided dental services to patients from the communities sampled.
Among the different communities sampled, seropositivity ranged from 4% (Jamaica) to 75% (Las Coaches; see Table 3).
Total richness among all midgut fungal communities sampled was 44 OTUs (Table 2).
The expression of several persistent OTUs in all beetle midgut communities sampled for sequencing was confirmed.
Interestingly, the same microbial communities sampled at different times or from different hosts can vary significantly.
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