Sentence examples for common reference sample for from inspiring English sources

Four self-self hybridizations were performed using the common reference sample for control of experimental background.

Microarrays were competitively hybridized according to Agilent specifications for two-color gene expression at 65 °C for 17 h, each tissue hybridized against the common reference sample for a total of eight microarrays.

Our experimental setup contrasted gene expression in the dauer stage (time point 0 hour) with the dauer-exit samples (time point 12 hour) via a common reference sample (mixed stage) for each species.

We followed the hybridization strategy used in the European Compendium of Arabidopsis Gene Expression project (http://www.cagecompendium.org), employing a Cy3-labeled mixture of oligonucleotides complementary to the secondary primers for probe amplification as a common reference sample and Cy5 labeling for the biological samples.

Further scaling the data to a common reference sample (herein referred to as total count scaling) introduces more variability by pushing all samples toward the same distribution, especially for samples with different sequencing depth.

For all the FF tumors and the Common Reference Sample, Agilent 244,000 feature whole genome microarrays were hybridized with tumor RNAs (Cy5) and a human common reference (Cy3) and lowess normalized as described in Herschkowitz et al. [ 30].

For example, using a common reference sample in combination with a test sample of type A and type B on one array allows a direct comparison of the relative gene expression levels in both test samples, without having to compare relative expression values from two or more dual-target arrays containing only a reference and one of the test samples.

Next, we normalized the mean expression/intensity data of each individual sample against the common reference sample in the computer program R (method 'aquantile'; version 2.14.0 for Windows, R Development Core Team, 2009).

For cDNA microarray and iTRAQ-based proteomics, a common reference sample was constructed by mixing equal aliquots from all six samples facilitating computation of relative expression profiles and expression ratios across tissues.

A common reference sample was constructed from a pool of all labeled RNA extracts.

The same common reference sample was thus used in all the 46 (2×23) hybridizations.