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Each amplified sample was hybridized twice against a common reference pool in a dye-swap experiment.
Intensities shown are the mean of all probes for each transcript (113 for ACC1 and 120 for ACC2), after normalization using a common reference pool.
For comparisons among TS and ES (R1) cells, siTcl1-transfected ES cells, ZHTc6 and ZHBTc4 cells, each sample was hybridized against a common reference pool of RNA.
The control samples were "a common reference pool of mRNA".
Protein quantities, relative to the common reference pool, were used as input when comparing proteomes.
The resulting "common reference" pool included all of the individuals used in the present microarray experiment.
Similar(37)
Using 50 µg of total RNA from prostate samples Cy5-labeled cDNA was prepared and Cy3-labeled cDNA used 1.5 µg of mRNA common reference, pooled from 11 human cell lines (see Ref. [792]).
Cy3-labeled organ and Cy5-labeled common reference (pooled samples from 6 organs) were co-hybridized.
Therefore, 800 ng total RNA were hybridized against a common reference (pooled RNA from all samples).
The 24 samples and a common reference pooled sample (made by taking an equal amount of protein from the EV 12 and EV 24 samples) were placed on eight 4-plex iTRAQ runs as described in Additional file 1.
The cRNA of the appropriate sample and the common reference (pooled RNA from all samples) were mixed and fragmented using the Agilent Gene Expression Hybridization Kit and transferred to the microarray slide.
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