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A new method, the common reference method, is proposed in order to overcome this problem.
Dual-color labeling with common reference method was used to hybridize the samples on Exiqon microRNA microarray version 11.
Without a common reference method and in the absence of a standardized assay, results varied considerably when the same sample was tested by different laboratories or methods or even when the same sample was tested repeatedly by one methodology.
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Our experiments include a comparison to common reference methods.
The results of this study indicate that there is a need to develop a universal Asian equation using standardized creatinine, and valid common GFR reference method.
Without a common reference, the current method will not be able to classify a single testing patient before the platform and protocol of microarray experiments are standardized.
In order to get an insight into the global structure of the complex dataset of this microarray investigation, we performed a principal component analysis (PCA) on a reduced dataset (see Methods) using the steady-state mRNA levels determined by the application of a common reference hybridization strategy (see Methods).
The predictive performance of the two models is compared to a common genomic BLUP as a reference method in a whole-genome simulation study considering various gene-action models.
Five percent differences are common if inappropriate reference methods are used, and laboratory comparison methods for blood glucose can have a total error of up to 10% [ 7].
The BWA aligner (Li and Durbin, 2009) and SAMTools (Li et al., 2009) were used to identify SNPs and INDELs in AT1, AT2 and UB1 against the common reference KW1 (see 'Materials and methods').
We performed hybridizations using direct-labeled randomly primed total RNA extracted from eight breast-cancer cell lines and one derived from normal breast epithelium against Universal Human Reference RNA as a common reference following standard protocols (see Methods).
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