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The experimental design (Fig. 1) was a common reference design for which the reference sample was an equi molar mixture of all the experimental samples and cDNA from the experimental groups was hybridized against this common control RNA sample.
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The strong correlation between arrays introduced by the common reference design of our experiment caused this variation to be common across all arrays (Additional file 13).
For example, when 3 treatments and a control are to be compared using 4 data points per comparison, 16 microarrays are needed for a common reference design, 12 arrays for a block design (treatment vs. control on an array), 8 when using a loop design, but only 4 with 4 dyes and the design shown in Table 4.
A common reference design was applied for the hybridizations, and the reference RNA sample was created by total RNA extracted from a balanced pool of 20 male and 20 female flies from each parental line.
A common reference design was used for this microarray experiment.
The common reference design was selected for this microarray experiment.
Common reference design was selected for microarray hybridizations.
For instance, in the case that four samples need to be compared in all combinations, a dual-label common reference design requires four arrays for a single analysis of each sample, whereas a four-label design would require no common reference because all samples can be hybridized on a single array and only one array for a single analysis of each sample is needed.
Our experimental design employed a common reference design, hybridizing three biological replicates for each time point [ 35].
The common reference design [ 23] was used for sample assignment in the dual color mode of expression assay on the Agilent Human Whole Genome 4 × 44k Microarrays to analyze ~40000 transcripts.
A reference pool was synthesized using equimolar amounts of Cy3-cRNA from each species/day/infection class condition to hybridize alongside experimental samples to control for hybridization difference in a common reference design [ 74] (19 samples used in reference pool).
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