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Whole-genome phylogenies were performed by concatenating all common alignment blocks found by Mauve in the comparison of the three B. henselae strains and B. quintana Toulouse (accession number: NC_005955.1).
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Also, the AU plots (Figure 1b) suggest that the common removal of signal peptides [17], [18], [19], [20] may not be necessary, since these areas still possess high quality alignment blocks.
We concatenated three alignment blocks, and manually excluded GAP sites and seemingly nonhomologous sites in the alignment (Supplementary Data 5c, d).
Large structural variations (>100 Kb) were identified based on synteny alignment blocks, with variation boundaries manually checked.
SNPs and indels were identified by show-snp from the one-to-one alignment blocks (parameter '-ClrTH').
Only one point is emphasized here: the modern world is in an era of common alignment.
We concatenated all alignment blocks for each dataset and used the resulting three alignments as input for SplitsTree.
We inferred individual MrBayes [30], [31] trees for each alignment block to see whether strain relationships varied between trees from different alignment blocks.
Alignment blocks of a minimum length of 40 were subjected to RNAz, utilizing an offset of 40 (for alignment blocks >80) and considering both DNA strands independently.
Resolving strain relationships may improve if regions of recombination were removed from the alignment blocks.
Concatenated alignment blocks from each dataset were imported into SplitsTree [27], [28].
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