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We identified, cloned and characterized a N. oceanica microsomal-like Δ12-desaturase (NoD12) mediating the committing step of LC-PUFA biosynthesis by converting oleic acid (18 1 n − 9) to linoleic acid (LA,18 2 n − 6).
MGAT1: committing step for synthesis of hybrid- and complex-type N-glycans.
This reaction is the first committing step in the pathway and catalyzed by Acetyl-CoA Carboxylase (ACCase).
The product of 4CL, p-coumaroyl-CoA, is the substrate of the enzyme chalcone synthase (CHS) [ 24], which carries out the committing step in flavonoid biosynthesis.
This is a committing step in the folate pathway because HP is the first compound in the pathway used only for the biosynthesis of folate cofactors.
* Contigs representing full length genes Oxidative decarboxylation of pyruvate to acety-CoA by pyruvate dehydrogenase (PDH) is the first committing step in fatty acid biosynthesis.
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Transcript profiling revealed coordinated increased transcript abundance for genes encoding enzymes of committing steps in the phenylpropanoid pathway.
The enzymes cystathionine γ-synthase (CGS) and cystathionine β-synthase constitute the committing steps in the transfer of the sulfur moiety from cysteine to methionine.
The inverse regulation of the two committing steps of phenylpropanoid metabolism, F3′H and F3′5′H, sharing a common substrate, was reflected by the metabolite profiles of the two parallel downstream anthocyanin pathways.
In addition, a series of genes encoding two enzymes of committing steps in the lipid biosynthesis pathway were examined as well: acetyl-CoA carboxylase (ACCase) and acyl-CoA, and diacylglycerol acyltransferase (DGAT), which catalyze the first and the final committed step in TAG biosynthesis, respectively.
We detect two events from commits: "commit" (step 5), i.e., committing an issue-resolving patch to a central repository, and "backout" (step 6), using the same heuristics we used in previous work (Souza et al. 2014, 2015).
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