Here, we observed that hypoxia exposure of mouse ESCs induced an initial phase of HIF1α-mediated upregulation of the transcription factor Etv2, which in turn induced the commitment to the EC fate.
Flowering plants have evolved a remarkable diversity of inflorescence branching patterns, which is largely governed by developmental decisions in inflorescence meristems and their derived meristems between maintenance of indeterminacy and commitment to the floral fate.
Conditional inactivation of the Sox9 gene at various times during mouse limb development also demonstrated that SOX9 is necessary for mesenchymal condensations, for the commitment to the chondrocyte fate at the time when the chondrocyte and osteoblast lineages segregate from a common progenitor, and for the overt differentiation of these cells into chondrocytes.
These results suggest that the irreversible commitment to the mesodermal fate occurred between days 4 and 6 after induction.
Our results show that Dnmt1 deficiency does not affect progenitors commitment to the photoreceptor fate, as Otx2 expression appears normal.
Rather, activation of this pathway is associated with exit from pluripotent state and commitment to the PrE fate.
Increased commitment to the OL fate was observed in ICI aTf post H/I as compared with H/I S animals (1.75-fold versus 1.35-fold increase relative to control, respectively; P<0.05).
These data demonstrate that PI3K/AKT inactivation stimulated melanoma GFPhigh cell differentiation, suggesting that TNF suppresses their commitment to the differentiation fate by activating AKT, consequently preventing asymmetric self-renewal and promoting symmetric self-renewal of GFPhigh melanoma SCs.
A combination of stimulatory signals, including Wnts, Sonic hedgehog (Shh), and Noggin, and suppressive signals, such as BMP4, ultimately dictate expression patterns of the primary MRFs, MyoD1 and Myf5, and therefore propel commitment to the myogenic fate.
Adipocyte differentiation can be viewed as a two-step process: (1) commitment to the adipogenic fate by mesenchymal stem cell progenitors and (2) induction of the adipocyte gene expression programme, which drives terminal differentiation.
