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Here, we show that the observed rate constant for commitment to fold to the native or misfolded states is 1.9 37 °C (37 °C, 10 mM Mg2+), the same within error as the rate constant for overall folding to the native state.
The ribozyme forms much of its tertiary structure significantly faster than it reaches this commitment point and the tertiary structure is expected to be stable, suggesting that the commitment to fold along pathways to the native or misfolded states is made from a partially structured intermediate.
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Do one load of laundry (time commitment: 3 minutes to start; 2 minutes to transfer; 5 minutes to fold).
To fold paper.
Know when to fold.
Hard to fold.
We began to fold.
Time to fold?
I hate to fold laundry.
Continue to fold cards.
Thus, the commitment to alternative folding pathways is made late in the folding process, concomitant with or after the rate-limiting step for overall folding.
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