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In the couple of years that followed, I passed a commitment boundary.
This insight facilitated transcriptional analysis of cells either side of the commitment boundary.
Modelling of this data set identified cells close to the commitment boundary and inferred a time course of commitment from static single-cell gene expression measurements 47.
Additionally, we have focused on capturing networks from closely related cells in the vicinity of the commitment boundary, to gain insight into the evolution of GRNs relevant to lineage specification.
While most of the work was performed in cell lines –as it would be difficult to capture cells at the commitment boundary in vivo – similar heterogeneity in the erythroid program was found in primary megakaryocyte-erythroid progenitors compared to more committed erythroid cells.
Similar(55)
Genome-wide targets have recently been described for many hematopoietic TFs (see Hannah et al., 2011), but studies have generally focused on binding within a single compartment, precluding appraisal of the lineage specificity of interactions and how cistromes change across commitment boundaries.
"It is a commitment without boundaries.
The abuser sees your lack of commitment to boundaries as a sign that you approve of their abusive behavior and in some way you are there to rescue them from themselves.
This example contains competing hypotheses that have commitments about boundaries and about natural selection.
The vote followed a bitter debate in which angry backbench Tory MPs repeatedly rounded on Liberal Democrats for backtracking on a commitment to a boundary review before the election.
It is not complete, but it is open and presenting a group show that demonstrates Mr. Schachter's commitment to blurring boundaries between art, architecture and fashion.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com