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Furthermore, our best catechol 1,2-dioxygenase gene variant resulted in 2.9-fold higher activity than a commercially optimized gene variant.
Specifically, eGFP expression was successfully optimized for high expression in rich media, and the CatA enzyme was optimized for high expression in stationary phase growth, with the highest expression variant resulting in 2.9-fold higher product yield over a commercially optimized variant.
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By doing so, we were able to design three CatA variants which outperformed both a commercially-optimized sequence (Blue Heron variant) and three control CatA variants in stationary phase.
In order to eliminate any undesired effect resulting from certain fusion partners of uncertain origin/source, we used commercially available, codon optimized fusion partner genes to construct the p3G, p3R, and p3H.
Reaction conditions were optimized with commercially available HRM mixes.
The RT Profiler™ PCR Array (SABiosciences Corp). is a commercially available set of optimized RT-qPCR primer assays on a 96-well plate.
Under the optimized conditions, 21 commercially available phenolic compounds and 25 commercially unavailable phenolic structures were analyzed in less than 30 min. The chromatographic method was designed as an alternative for the provisional identification of these compounds before their full characterization.
Understanding the switch to secondary metabolism is of major importance in biotechnology, where it can contribute to the optimized production of commercially relevant secondary metabolites, such as antibiotics.
Since the 1970s, recombinant DNA technology has revolutionized the ability to engineer microorganisms by modifying specific genes and pathways for optimized production of commercially significant metabolites [ 78].
The performance of the TDM was sufficiently optimized to make it commercially viable.
The protocols that are presented for array fabrication, quality control, labeling, and hybridization utilize both "home‐brew" and commercially available products to achieve an optimized set of cost‐effective tools.
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