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The cells were fixed and stained with fluorochrome-labeled antibodies to CD14 to gate on monocytes, and co-stained with commercially labeled antibodies to phosphorylated forms of MAP kinase proteins: phospho-p38, phospho-p44/42 Erk kinase and phospho-Jun kinase (JnK).
A 1930 painting entitled Tp 2, whose blue plastic support was long described in museum records as Trolitan (the German version of Bakelite), turned out to be a cellulose nitrate substance commercially labeled as Trolit F which, unlike Bakelite, is easily prone to degradation.
The purity and immunophenotype of immature and mature MDDC was assessed by flow cytometry analysis using commercially labeled monoclonal antibodies (mAbs) against surface markers (See below, "Flow Cytometry").
The purity and immunophenotype of mature MDDC were assessed by flow cytometry analysis using commercially labeled monoclonal antibodies (mAbs) against surface markers (see below, "flow cytometry").
As a preventive strategy, raloxifene may have more appealing characteristics compared to tamoxifen, since it has a better safety profile and is not commercially labeled as an anticancer drug; both are important aspects to consider in preventive care.
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E. coli, commercially labelled with fluorescein isothiocyanate (FITC) (Orpegen Pharma, Heidelberg, Germany), was added to aliquots of blood from each lithium heparinised blood sample and incubated for 10 minutes at 37°C.
The immunobead RT-PCR technique was performed as described above using the beads commercially labelled with the BerEP4 antibody, CELLection Epithelial Enrich Dynabeads (Dynal, Invitrogen), to ensure optimal isolation of circulating HNSCC expressing the EpCam protein.
Quantification was achieved by isotope dilution in all cases by reference to commercially available labeled isotopes [ 12].
In this case, the labeling can be controlled through the Wittig reagent, 3c, which was synthesized from commercially available labeled ethyl bromoacetate (1c) depending upon the desired labeling.
Oligonucleotides were synthesized commercially (Metabion), labeled with fluorescein isothiocyanate (FITC) and tested for specificity against the set of reference organisms listed above.
For flow cytometric analysis we used the following commercially available labeled mAbs directed against human CD markers: anti-CD3 with PerCP or Alexa Fluor 700 label (BD Biosciences, San Jose, CA, USA), allophycocyanin-labeled anti-CD4 (DakoCytomation, Glostrup, Denmark) and biotinylated anti-CD8 (Serotec, Düsseldorf, Germany).
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