Detection of HCV antibodies was performed by a commercial enzyme immunoassay test of third-generation (ORTHO HCV 3.0 – commercialized by Dia Sorin, Saluggia, Italy) following the manufacturer instructions.
Droplet-based strategies have also been commercialized by Bio-Rad Laboratories and RainDance Technologies.
Commercialized by Cell Microsystems, MRAs typically contain about 10,000 wells, Allbritton says, though some measure in the millions.
Johnsson has likewise developed rhodamine variants that he calls "SiR dyes" (carboxylated silicon-rhodamine) that are commercialized by Spirochrome.
Magnetic Insight, for instance, is advancing a method called "magnetic particle imaging" (MPI; also commercialized by Bruker).
SPR is a mature technology that has been commercialized by companies such as Biacore (now part of GE Healthcare) to quantify protein-protein and protein-ligand interactions.
Lisa Ellerby at the Buck Institute for Research on Aging reprograms her cells by delivering purified transcription factors, a strategy that, like mRNA-based reprogramming, has been commercialized by Stemgent.
Published in 2013, Zhuang's study is "a seminal contribution," agrees Joerg Bewersdorf, Associate Professor of Cell Biology and Biomedical Engineering at the Yale University School of Medicine, who builds superresolution microscopes (including one now commercialized by Bruker) in his lab.
Like flow cytometry, mass cytometry, commercialized by DVS Sciences as the CyTOF (recently acquired by Fluidigm), is a flow-based method that uses heavy metal-conjugated antibodies rather than fluorescent dyes to achieve its high information content.
That's why he has edged away from pure superresolution toward ultrahigh-speed imaging with a new (albeit superresolution-compatible) design called "lattice light-sheet microscopy," soon to be commercialized by Zeiss and 3i. .
Other RNA-based strategies include a self-replicating RNA developed by Steven Dowdy at the University of California, San Diego (and commercialized by both EMD Millipore and Stemgent) and Sendai virus, available from Life Technologies/Thermo Fisher Scientific.
