Sentence examples for commercial sequence from inspiring English sources

About 200-base genomic regions around the SNPs and indels called by the mapping programmes were amplified by PCR and sequenced on a capillary sequencer with the Sanger method using the commercial sequence service of MACROGEN (Tokyo, Japan).

Purchasing a commercial sequence analysis suite is not as simple as a one-time payment followed by a lifetime of bioinformatics bliss.

The first DNA microarray for P. pastoris was produced using commercial sequence data (Integrated Genomics, Chicago, IL, USA), containing partial P. pastoris genes, and examined the unfolded protein response during protein production [ 6].

Commercial sequence analysis suites, in addition to assembling and mapping NGS data, are designed to carry out the day-to-day bioinformatics tasks involved in molecular, evolutionary and genome biology.

Purified amplicons were sequenced using both L2 and U2 primers by a commercial sequence provider (Macrogen) using the Big Dye terminator cycling (Applied Biosystems) condition and analysed by the automated sequencer ABI 3730XL.

PCR products were purified using the QIAprep Spin Miniprep Kit Qiagen Ltd , UK) according to the manufacturers instructions and sent to a commercial sequence service provider (MWG Biotech, Germany) for sequencing.

PCR products were sequenced directly using the amplification primers using Big Dye v3.1 on a 3730xl automated sequencer (Applied Biosystems, Foster City, CA, USA) by a commercial sequencing service (Macrogen, Seoul, Korea) and a MegaBace 1000 capillary instrument (GE Healthcare, Wakeusha, Wisconsin, USA) using Dyenamic ET terminators (GE Healthcare) according to the manufacturer's instructions.

Bidirectional resequencing was performed using a commercial sequencing kit (ABI BigDye Terminator Sequencing kit V2.1; Applied Biosystems) and an automated capillary sequencer (ABI 3730; Applied Biosystems).

Using Shannon's entropy, we objectively measured the HLA typing ambiguity of various commercial Sequence-Specific Oligonucleotide (SSO) protocols (groups of SSO kits used in tandem).

Plasmid DNA was isolated and sequenced by commercial sequencing company (Sangon Biotech).

DNA loading and trapping in nanocavities is important for various applications including commercial sequencing applications57.