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RNA was extracted following the commercial protocol with an Invitrogen Pure Link RNA mini kit and quantified by absorbance at 260 nm.
SVF was collected from all patients, and processed using a commercial protocol which has been used in veterinary medicine for >2 years.
The cells were transfected with commercial protocol provided by the manufacturer.
Purified protein cleaved from beads using Prescission protease was then purified by FPLC and utilized to inoculate rabbits in a commercial protocol carried out by Cocalico Biologicals.
To estimate transcript mutation rates (Table S2), RT-PCR products were cloned into plasmid following a commercial protocol (PCR-Script™ Amp, Stratagene) and sequenced by standard automated sequencing after isolating DNAs from plasmids by alkaline miniprep kits (Qiagene).
For muscle tissue, we extracted DNA using the DNeasy Tissue Kit and a commercial protocol (Qiagen, Valencia, CA, USA).
Similar(42)
Since the library products are quite short, there is fairly limited capability to do this without modifying more commercial protocols.
Different commercial protocols for miRNA library preparation have been developed.
First, most commercial protocols produce RNA only in the antisense orientation.
DNA was extracted from either frozen blood or tail tissue using commercial protocols (Gentra Systems, Minneapolis).
DNA was labeled according to commercial protocols using the IrysPrep Reagent Kit (BioNano Genomics, Inc).
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