Total RNA was extracted using a commercial kit (miRNeasy microKit, Qiagen, Hilden, Germany).
DNA extraction was carried out using a commercial kit (Gentra Puregene Buccal Cell Kit, Qiagen).
Commercial kit classifications were correct for nine out of ten samples.
However, KIR sequencing-based typing (SBT) commercial kit is not available.
At present, a commercial kit for GBV-C RNA quantitation is not available.
A commercial kit was used according to the manufacturer instructions (Promega, PROM G35800001, Lisbon, Portugal).
Plasma insulin levels were measured using an ELISA commercial kit.
Phospholipid content was determined using a commercial kit (Wako Chemicals).
Samples which tested negative with the commercial kit and positive by L-asparaginase ELISA were re-assayed with a second commercial kit for Helicobacter pylori IgG (commercial kit 2).
We stratified by type of commercial kit since they have standardized protocols.
Ribonucleic acid (RNA) was isolated using a commercial kit (RNeasy mini kit, Qiagen Inc., Valencia, CA).
