Sentence examples for commercial buffers from inspiring English sources

Act as commercial buffers since transformations and supply chain services are provided while goods are inside the free port.

pH-measurements involved calibration of the pH-measurement device (Orion pH-meter. and Thermo-science Fisher electrode) with four commercial buffers.

Intracellular staining was performed with commercial buffers (Cytofix, BD Biosciences; Perm buffer III) for rat CD2, TCR β, phospho-ERK and FoxP3 detection.

Dynabeads were incubated with lysates for 4 h at 4°C, and washed consecutively with commercial buffers (Low Salt, High Salt and LiCl Immune Complex Wash Buffers; Upstate).

The PCR reactions contained 0.2 μM of F1.2 and R1 primers, 1 U proofreading Ex Taq DNA polymerase (TaKaRa), and 1X each of the following premixed commercial buffers: GCI and GCII with 2 mM MgCl2 (TaKaRa), and Buffers D, E, F, G, H, and I (Epicentre™).

As reference we extracted cryo tissue with the commercial buffer T-Per (Thermo-Fisher, Rockford, USA), a dedicated cryo extraction buffer, according to the manufacturer's instructions.

The performance of our in-house buffer was as good as that of the commercial buffer and it consistently yielded similar results.

PCR amplification experiments were performed with the Expand High Fidelity Plus PCR System (Roche) in a final volume of 50 µl of commercial buffer containing 1.5 mM MgCl2.

3 µg aliquots of DNA were digested overnight with 30 U of Alu I, Hpa II or Hae III in the corresponding commercial buffer (New England Biolabs).

Comparative protein extractions from fresh frozen tissues were performed with both buffers (EXB Plus and EXB) and with another commercial buffer for protein extractions for fresh frozen tissue (T-Per, Thermo-Fisher, Rockford, USA).

Retinal tissue (pooled from 4 eyes for each time-point) was lysed using a commercial buffer (RIPA, Sigma Aldrich, Gillingham, UK) with added protease inhibitor cocktail (Sigma Aldrich, Gillingham, UK).