Vancomycin concentrations were determined using a commercial assay (Cobas C Systems Roche).
Caution is advised in using this type-specific commercial assay for clinical purposes.
The contents of serum TNF-α and TGF-β were determined by commercial assay kits according to manufacturer's instructions.
The assay was more specific, and at least as sensitive as a commercial assay, and allowed direct sequencing of amplicons.
These results were in statistical agreement with those obtained by commercial assay of enzyme-linked immunoassay kit (ELISA).
Murine tumor necrosis factor alpha (TNFα) or human TNFα, concentrations were determined using commercial assay kits (DuoSet ELISAs; R&D Systems, Wiesbaden, Germany) according to the manufacturer's instructions.
Total protein, albumin in egg white and cholesterol concentration in egg yolk were determined in the Humalyzer, Microlab 200 using commercial assay kits (Biolabo, France).
The newly developed ELISA is based on full-length cN-1A antigen and represents the first fully standardized commercial assay for routine anti-cN-1A testing.
Supernatant magnesium concentration was measured colorimetrically using a commercial assay kit (Nanocolor Härte 20, Macherey Nagel, Düren, Germany) as previously described (Schumacher et al. 2011).
GSH-PX, TAOC, TNF-α, TGF-β, lipase and trypsin were detected by commercial assay kits purchased from the Nanjing Jiancheng Bioengineering Institute (Nanjing, China).
Cathepsin B activity was measured by commercial assay (BioVision) according to manufacturer's protocol.
