Exact(3)
Specific comments: Methods Long PCR has been widely used for specific amplification of CYP2D6, with reaction conditions and primer sequences well established.
Therefore we would like to keep these sentences in their current location as a means of clarifying the use of the data.Reviewer comments:Methods· In the first paragraph, the sentence: "In general…(see diagram)." Is unclear.
Main comments: Methods The authors should comment on the use of the ROCK inhibitor Y-27632 in their protocol for cell sorting and during subsequent plating at low density.
Similar(57)
Both of latter commented methods, very used in this field [4, 44], provide good solutions to indexing and description [44].
We would like to highlight that the commented method to obtain the initial guess properly worked in all the simulated and real data available to the authors.
Comments: All methods assessed showed poor accuracy.
For simplicity of explication, the person is used as the final unit of analysis in this chapter; yet, virtually all the comments and methods extend to the household or family.
LB provided valuable comments on methods and helped on editing.
Statements in this category included comments about methods for searching or navigating.
AM, SG and KM wrote part of introduction and discussion, and contributed with ideas and comments to methods and results.
The article is very well written and I have only a few minor comments: The Methods section is missing.
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