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All animals were introduced to hydrated diet (prepared as described above) 24 36 h before commencing the experiment.
The surface soil in the plot area in Fedis site before commencing the experiment is a silty clay loam, containing 36% sand, 45% silt, and 19% clay.
In general, after reaching the desired temperature the larvae were kept undisturbed for the next 36 h before commencing the experiment and after this a batch of 30 larvae was introduced into each respective respiratory chamber.
Chambers containing 1 refuge and 1 tadpole from the appropriate treatment (Bd+ or Bd−, and predator cue were left overnight to acclimate before commencing the experiment.
Mice were age matched, aged 8 10 weeks old when commencing the experiment.
The first signal emerged ca. 2 h after commencing the experiment, signifying the start of crystallization.
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The initial tension was adjusted to 1.5 g and an equilibration period of 90 min was allowed before commencing the experiments.
Before commencing the experiments, the participants were instructed to stop looking at the rubber hand and start looking at the knife as soon as it was presented to make sure that they perceived the threat.
Ten minutes before commencing the experiments, each bee was checked for intact PER by touching one antenna with a toothpick that had been dipped into 1M sucrose solution without allowing feeding.
The animals were allowed to acclimatize for 5 days before commencing the experiments.
Animals (n = 5 sham and n = 5 sepsis) were fed with a specific manganese-reduced diet over a four-week period prior to commencing the experiments (to avoid interference by the near-infrared properties of manganese).
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