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Before its commencement, the study was approved by the Research Ethics Committee of China Medical University, and informed consent was obtained from each participant.
Prior to commencement, the study received approval from the Human Research Ethics Committee-Northern sector, South Eastern Sydney Local Health District, New South Wales Health.
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Ethical approval was obtained from the relevant ethics review boards prior to commencement of the study.
Animals were kept for 5 days to allow for acclimatization to the laboratory conditions before commencement of the study.
The institution's ethical approval was obtained before commencement of the study, and all randomized patients gave informed consent.
All fish were acclimated to laboratory conditions for a minimum of 12 days prior to commencement of the study.
Rats were arranged into four treatment and one control group at the commencement of the study as shown in Table 1.
Prior to the commencement of the study, the animals were allowed to adjust and adapt to the new environment for a period of 3 weeks [9].
All surgeries were performed by author KE and all required approvals were obtained from MCPHS University IACUC committee prior to the commencement of the study.
All subjects gave informed consent before commencement of the study.
Regional Ethics Committee approval was granted before commencement of the study.
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