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"A true team leader... Definite command potential.
The EPSC decay time constant, but not its voltage dependence on the command potential, was sensitive to the holding potential.
Time constants for activation and deactivation were determined and plotted as a function of command potential (Fig. S1), showing that β4 slowed both the activation and deactivation time course.
The quadrature lock-in signals, as well as TEVC ionic current, membrane potential and applied command potential were recorded through a 16-bit A/D converter ITC-16000, HEKA Inst. Bellmore, NY).
(C ) As an approximation of the voltage-dependence of inward current activation, currents at each command potential from A were converted to chord conductance estimates assuming ENa = 75 mV.
Boltzmann fits yielded, for control solution, Vh = −10.1 ± 0.4 mV with z = 4.1 ± 0.4 e ; with 20 mM Cs+, Vh = −15.9 ± 0.2 mV with z = 3.7 ± 0.1 e. (D ) Mean Nav conductance was plotted as a function of command potential for 10 dKO DRG neurons and fit with a Boltzmann function.
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To obtain the I V curve of CaL, the current densities were plotted against the corresponding command potentials.
From the EPSCs recorded at the different command potentials the I V relationship was derived and the ACh reversal potential (EACh) that prevailed over the range of physiological membrane potential values here considered (−30/−100 mV) was estimated by extrapolating the Goldman current equation fitted to the whole data set (see below).
The average decay constant significantly decreased when currents were elicited from hyperpolarized neurons: the mean time constants in neurons held at −40 mV were 6.3±0.5 and 8.2±0.6 ms for −40 mV and −90 mV command potentials, respectively, whereas in neurons held at −90 mV the corresponding values were 4.8±0.4 and 6.4±0.5 ms (n = 10).
Thus, single-channel conductances were usually calculated at positive command potentials.
Although net outward current was generally similar in both groups, the dKO cells exhibited a larger outward at command potentials from −10 mV and more positive.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com