Sentence examples for combined with buffer from inspiring English sources

Instead of operating an effusive source at room temperature it can be combined with buffer gas cooling technologies.

EMSAs were performed as follows: P-labeled DNA (~0.5 ng, 50,000 cpm) and 2 μg of nuclear protein were combined with buffer (final concentrations: 10 mM Tris-HCl, pH 7.5, 1 mM EDTA, 100 mM sodium chloride, 0.5 mM magnesium chloride, 1 mM DTT, 20 μg BSA, 10% glycerol, 0.5% Triton X-100) and poly dI-dC (1 μg) in a final volume of 20 μl.

We prepared a 6 × stock of 1.5 mM 12-pNCA in 36% dimethyl sulfoxide (DMSO) and the EPPS buffer (the 12-pNCA was stored in the DMSO solution and combined with the buffer immediately before use).

A hat oblique tunnel portal combined with a buffer structure with top holes was particularly effective.

One µg of each RNA sample was combined with loading buffer, and gels subject to electrophoresis at 80 V for 40 50 min at room temperature.

For Illumina arrays, biotin-labeled cRNA (750 ng total RNA-cRNA or 2 µg NRO-cRNA) was combined with hybridization buffer + formamide and hybridized (16 hr) at 55°C to Illumina's Sentrix HumanRef-8 Expression BeadChips (Illumina, San Diego, CA, Cat#11201828).

Biotin-labeled fragmented cRNA samples were combined with hybridization buffer containing hybridization Control cRNA and Control Oligo B2 (Affymetrix), before hybridization to GeneChip® Xenopus laevis Genome Array for 16 h at 45°C.

Samples for comparison were mixed in pairs in order to carry out a direct competition hybridization experiment, then combined with SlideHyb Buffer (Ambion), mouse Cot-1 DNA (Invitrogen Corp ., RNA polyA (Sigma-Aldrich, St Louis, MO), and yeast tRNA (Invitrogen Corp .. Samples were subsequently hybridized to CNIO mouse cDNA microarrays.

Leaf tissue was ground in liquid nitrogen and the powder combined with homogenisation buffer.

For detection of versican, samples were combined with sample buffer without reducing agent.

Next , 25 μL of plasma was combined with assay buffer in the well of a 96-well plate.