Fragments were combined to a consensus sequence with the GENEDOC sequence editor (www.nrbsc.org/gfx/genedoc), and deposited in GenBank (accession no. FJ528908).
The symbols associated with the vertices are combined to a so-called PhAST-Sequence beginning with the vertex with the lowest canonical label.
All information is then combined to construct a sequence template for a primer design program.
The two searches were combined to produce a sequence similarity network based on transitivity clustering using the plugin Blast2SimilarityGraph in Cytoscape (Srinivasan and Moon, 1999; Shannon et al., 2003; Wittkop et al., 2010).
When there was a single cluster representing a single gene family, the constituent ESTs were combined to create a single sequence, which was defined as the potential gene sequence (pHap) for that family.
Therefore, DNA data obtained from 4 patients was combined to generate a consensus sequence heretofore designated the ZIKV 2007 epidemic consensus (EC) sequence (GenBank accession no. EU545988).
We then used a Perl script to generate a reference sequence for each dataset that identified all retained indel and SNP positions using IUPAC codes, and these were combined to create a comparable sequence for Douglas-fir.
Nile Red was excited using the 488 n M laser and the cells photographed every 5 min for 2 h in the z-axis and combined to give a continuous sequence.
As implemented here, multiple pools of BAC clones from a minimum tiling path (MTP) across a region can be sequenced, assembled, and combined to generate a high quality draft sequence of a chromosome arm and, by extension, can be applied to a whole genome in a straightforward manner.
The translated sequences of the 5' and 3' clones were then combined to produce a full-length sequence.
