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Recent studies of genomic and functional datasets based on combined sequencing data and established CENH3-associated DNA sequences revealed a more detailed insight into genomic architecture of centromeres.
The other teams that combined sequencing data were the CBCB team that used all three types of data and the ABL team which used Illumina plus Roche 454 data.
Figure 4 shows the number of times that each mutation was observed in the combined sequencing data for the three biological replicates; Figure 4 figure supplement 1 shows the same data for the replicates individually.
DOI: http://dx.doi.org/10.7554/eLife.03300.010 In contrast, only 53% of multi-nucleotide codon mutations are observed at least five times in the combined sequencing data for the mutant viruses (mutvirus samples), and only ≈ 26% of such mutations are observed at least five times in sequencing of the mutvirus for each individual replicate.
More than 99.5% of multi-nucleotide codon mutations are observed at least five times in the combined sequencing data from the plasmid mutant libraries (mutDNA samples), and ≈ 97.5% of all such mutations are observed at least five times in sequencing of the mutDNA for each individual replicate.
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The neighbor joining (NJ) method with uncorrected ("p") genetic distances was used to analyze combined sequence data for all 142 isolates.
Combined sequence data were analyzed under three different weighting schemes using Bayesian methods.
Phylogenetic relationships among lineages of Pyura stolonifera were reconstructed using an aligned data-set from combined sequence data of four loci, totaling 2611 bp in length.
When Rokas et al. [ 68] combined sequence data derived from a set of different genes, conflicting signals from individual gene sequences were resolved and the resulting phylogeny was strongly supported.
We combined sequence data from genome projects for M. truncatula, Oryza sativa, Populus trichocarpa and A. thaliana, as well as ESTs from the TIGR Gene Indices [ 49], and TIGR Plant Transcript Assemblies [ 50] from legume species and various plants.
We combined sequence data from these five mtDNA genes with nuclear rDNA 28S to form a dataset designed to address the following research questions: (1) What is the population structure of P. badia?
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