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For the combined sequences, the genetic distances among the primary lineages varied from 1.4 to 2.2 % (Table 4).
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It then coalesces x and y into a combined sequence the D value of which is computed as the maximum (instead of the average) of those of x and y.
Furthermore, we calculated the Ks for the combined sequences of the three nuclear genes and the Kimura two-parameter distances for the nrITS and chloroplast fragments (Additional files 2, 3 and 4).
A Neighbor-Joining tree based on the number of substitutions per site under the Jukes-Cantor model was constructed from the combined sequences of the 152 Rupicapra individuals.
The combined sequences of the two BACs are 77.7 kb long and contain a total of 38 predicted genes (028F08-082I18_1 to 028F08-082I18_38).
Because we mainly used the combined sequences to reconstruct the tree, we also calculated base composition for the 12 protein-coding gene set (Additional file 3).
Due to the low number of indels in the introns the combined sequences could easily be aligned by eye.
Despite the large differences in the length of the combined sequence between the two alignment methods (mostly due to LSU), the changes in the topology or level of support of the resulting trees were negligible (see below).
The combined sequences from all treatments were assembled into the gene-equivalent isogroups and the plausible splice variants, isotigs.
The phylogeny of cyprinid fish species was obtained from the molecular analyses of the combined sequences of cytochrome b, 16S and control region of mtDNA [ 54].
Our analyses are based on both the combined sequences of two genes, but when each gene was analyzed independently, the results were very similar (data not shown).
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