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To be able to understand and to control heparosan polymer elongation using PmHS2 glycosyltransferases, the effect of combined parameters on PmHS2 polymerization process were investigated using a fractional factorial design with four variables (three levels (34 − 1) for each variable).
Multifactor analysis indicated significant effects of all individual parameters (time, treatment and tissues) on actin and big defensin expression and a significant effect of combined parameters on AP-1, lysozyme and TLR expression (Table 4).
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Exponential phase cells (equivalent to OD600 nm 1) were exposed to 0-400 mM of Cs (a) or Sr (b) for 24 h, stained with propidium Iodide (PI) and thiazole orange (TO) and were gated on combined parameters of FSC, SSC and FL2 (see text for details).
The classification of lesions as malignant or benign on PET/CT was based on combined parameters from both PET and CT.
For each subject that showed transition in albuminuria, a matched control subject was selected that most optimally resembled the case subject on these combined parameters.
Based on these combined parameters, subgroups of patients with Dukes' stage B and C colorectal cancer could be identified as having either a high or a low risk regarding overall survival.
This selection criterion is a combined parameter based on a time scale and sensitivity analysis, identifying both short lived species and species with respect to which the observable of interest has low sensitivity.
Using the combined parameter estimates over all conditions, the mean fitted time series was also computed and, from the combined bootstrap parameter estimates for each bootstrap, the 95% confidence limits on the fitted time series was computed.
Final model parameters are summarized in Table 2. Whenever possible, the model was reduced by combining effect parameters on different transfer rates.
The quantitative risk analysis is performed after selecting key parameters and the probability and consequence of all individuals risk combined on parameters affecting the project financial performance and cash flows.
The surface resistance parameters are often combined into one parameter, the 'bulk' surface resistance parameter which operates in series with the aerodynamic resistance.
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