Exact(5)
A combined directed evolution and computational design approach is becoming increasingly important in exploring enzyme sequence-space and creating improved or novel enzymes.
This paper describes the recombinant expression and characterization of a new and highly active cold-adapted xylanase from the GH-family 10 (Xyl-L), and the use of a novel one step combined directed evolution technique that comprises saturation mutagenesis and focused epPCR as a feasible semi-rational strategy to improve the thermostability.
The qualitative analysis of the answers combined directed, deductive content analysis and conventional, inductive content analysis [ 24, 25].
Using combined directed and random mutagenesis approaches, the researchers generated several variants of eqFP611, the two most interesting being RFP611 and RFP639, where the number indicates the emission maximum of each protein.
In another study, a special structure of support vector machine (SVM) was proposed, which combined Directed Acyclic Graph-Support Vector Machine (DAG-SVM) with recursive undecimated wavelet packet transform, for inspection of broken rotor bar fault in induction motors [13].
Similar(55)
By combining directed and focused molecular evolution, a novel, strongly expressed alkaline laccase variant was identified.
Thermostable laccases with a high-redox potential have been engineered through a strategy that combines directed evolution with rational approaches.
Here, we review the potential of combining directed evolution with process engineering, and recent developments to improve their implementation.
Here, we tried to assemble an unimpeded biosynthesis pathway by combining directed evolution and metabolic engineering in S. cerevisiae for lycopene-overproduction.
By combining directed evolution with more global evolutionary engineering strategies, it may be possible to simultaneously co-evolve multiple proteins in this process.
In fact, she has only recently engaged an agent again, as she combines directing commitments with singing on the international cabaret circuit.
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