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The two dye-coupled samples for each array were combined and fragmented using 4 µl Fragmentation Reagents (Ambion).
One microgram aliquots of Cy3-labelled cRNA were combined and fragmented in a hybridisation cocktail (Agilent Technologies).
Following purification, 1.6 μg of Cy3 dye-labeled cRNA, as measured by NanoDrop 2000c spectrophotometer (Thermo Scientific, Bellerica, MA), were combined and fragmented.
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Fragments were combined and double digested with EcoRI/HindIII (NEB) and ligated (Epicenter).
PCR fragments were combined and used as a template in second overlay PCR using primers 41_Prom_Yap1F and 41_mGFP_R.
The generated DNA fragments were combined and 1/10 part by volume 7 m NH4Ac and three parts by volume 96% EtOH were added to precipitate the DNA.
The amplified fragments were combined and concentrated to 60 μl using Microcon ® YM-30 Centrifugal Filter Devices (Millipore Corporation, Bedford, MA, USA).
The template fragment is rotated by θ1-θ2+180°, abouthehe atom N. The two fragments are then combined, and the result recorded.
MetaML allows the programmer to construct, combine, and execute code fragments in a type-safe manner.
In the paper, we combine two measures "errors corrected" and "fragments cut" and propose a new optimization model, called Balanced Optimal Partition (BOP), for single individual haplotyping.
Among the diploid species of A. millefolium agg., gene trees of the two nuclear loci, ncp GS and SBP, and the combined plastid fragments are incongruent with each other and with the AFLP tree likely due to incomplete lineage sorting or secondary introgression.
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