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18. Prepare p-nitrophenyl phosphate solution in dH2O and mix thoroughly until fully dissolved.
Mix the coffee and sugar until fully dissolved.
5 Stir in the sugar until fully dissolved.
Whisk in sugar and milk powder until fully dissolved.
Stir until fully dissolved and then adjust to pH 7.6 by 2N HCl).
The solutions were mixed, tightly capped, and left to sit until fully dissolved.
The samples were heated and sonicated until fully dissolved with gels forming upon cooling to room temperature.
Stir for 2 minutes until fully dissolved.
Then mix until its fully dissolved.
Mix the honey with the vinegar until it is fully dissolved.
This mixture was gently pipetted until the pellet fully dissolved, typically in 30 seconds.
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