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By using the combine methodology of accutase pretreatment and microporation, we have been able to achieve high transfection efficiency without affecting human islet survival and function.
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Our combined methodology is readily applicable in combination with advanced MPC methodologies such as multi-parametric MPC (MP-MPC) (Pistikopoulos, 2009).
Fig. 5 Proposed workflow for the new combined methodology.
It was therefore taken as the only input for the combined methodology.
The combined methodology is generally applicable to other human infectious viruses.
For clarification on the performed steps, the proposed workflow for the combined methodology is displayed in Fig. 5.
To illustrate this combined methodology we have used stem-analyses data of four Sitka spruce (Picea sitchensis (Bong).
The objective is to explore the efficiency of this combined methodology.
This paper proposes to implement a combined methodology to tackle these estimates.
As a result, the combined methodology followed here takes advantages of both exergy and pinch analyses.
A new combined methodology for computer aided molecular design and process flowsheet design is presented.
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