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SOD3 reporter plasmids (300 ng), pCMV β-gal plasmids (50 ng), and specified combinations of wild type and mutant siRNAs (combined concentration of 100 nM) were transfected using Lipofectamine 2000 (Invitrogen).
Note in Supplementary Table S1 that this result for C = 6 is unchanged for all combinations of wild type mutation rate, cell generations to cancer, and degree of fitness increase within the explored parameter values.
We transplanted combinations of wild, domesticated and hybridized populations of a fish species to new environments.
These reporter plasmids were co-transfected with various combinations of wild type and mutant siRNAs for Sox10.
To determine optimal conditions for Tau-aggregation, we used different combinations of wild type Tau and mutant TauP301L forms as inducer and receiver.
Combinations of wild type and phosphorylation deficient GIRK1S385S401CT407C and GIRK4T199CS412C subunits were coexpressed and their regulation by cAMP injections was compared.
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As part of an ongoing effort to understand mechanisms contributing to P. aeruginosa pathoadaptation, we constructed a set of isogenic PAO1-derived strains bearing different combinations of wild-type and mutant mutS and dinB alleles.
Sand fly infection experiments were repeated four times for combinations of wild-type (FVI), KO and Kin lines and twice for combinations of FVI, +HASPB and +SHERP lines.
To find out how the two spatially separated RING domains work together to catalyze ubiquitination, we constructed a set of TRIM25 dimers with different combinations of wild-type and truncated monomers.
To further examine the redundancy between the two ptCpn60β proteins with regard to plastid division and greening, we observed all possible combinations of the ptcpn60β1-1 ptcpn60β1-1β2 mutandons (i.e. combinations of wild-typtcpn60β2ozygous and homutationsmutations).e
Whole-cell, GABA-activated currents were recorded from HEK-293 cells co-expressing different combinations of wild-type (WT) and/or mutant α4(L297S), β3(L284S) and δ(L288S) subunits.
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