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All possible combinations of genomes from 1 to M (the maximal number of available genomes) were calculated.
The recombinant pool would have started with all combinations of genomes, some with left end promoters A1-A3 present, others with those absent, and similarly for Plac.
Orthologs shared between species were identified with amino acid sequence searches between all pair-wise combinations of genomes using USEARCH [ 34].
To estimate the relevance of this particular number of shared proteins, we performed a similar analysis to identify proteins shared between all possible four combinations of genomes, while being absent in the remaining three genomes.
For each number of organisms considered (2 27 for Listeria sensu lato, 2 10 for Listeria sensu strictu), the inferred sizes of core and accessory genomes were recorded for 10,000 replicates of randomly selected combinations of genomes.
To discriminate mixtures of genomes at various dosages, the profiles of fragments and band intensities were initially established by sequential mixtures of DNA samples from the M. acuminata (AA; 'Calcutta 4') and the M. balbisiana (BB; 'Butuhan') genomes to obtain various artificial combinations of genomes.
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The size of the accessory and core genomes for different numbers and combinations of genome comparisons was calculated from the genome alignment.
Specifically, sets of P450s from each genome were reciprocally blasted [ 32] in all combinations of genome pairs using BlastP program with an E value cutoff of 1E-50.
The segmented nature of rotavirus genomes enables reassortment events in which novel rotavirus strains are produced with new combinations of genome segments derived from parental virus strains (3 ).
We have recently used combinations of genome-wide ChIP and DamID data to generate high confidence in vivo binding profiles and define a set of core binding intervals for both Dichaete and SoxN in Drosophila melanogaster.
Given that reassortment generates novel combinations of genome components derived from different species or strains, the diversity of combinations that are observed in a population (hereafter referred to as genome constellation diversity) can provide an indication of the frequency of past reassortment events (Dugan et al. 2008).
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