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Microarrays containing combinations of extracts and recombinant allergens will likely be the most beneficial diagnostic approach, combining comprehensive testing and protein-specific analysis, as suggested by Fall and colleagues [8].
The combinations of extracts were necessary as HGFIN is a membrane molecule [ 1]. Cell membrane extracts were obtained as previously described [ 17].
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Therefore, the combination of extracts decreased the toxicity on the liver and kidney of the mice.
This combination of extracts has been characterized pharmacologically in vitro and in vivo [ 8].
Not only does this procedure result from the combination of extracts, but also it is due to combinations of natural and synthetic products or antibiotics [ 55, 64, 65].
The combination of extracts from Herba Epimedii and Fructus Ligustri Lucidi demonstrated potential as a protective treatment for retinoic acid-induced osteoporosis in rats.
The B16F10 cells were exposed to α-MSH (100 nM) for 1 day and treated with a combination of extracts and α-MSH for 2 days.
The combination of extracts in this study may be an alternative approach to statins in people who cannot or do not want to be treated with statins.
Notably, lower IC50 value indicated higher synergistic effect of the combination of extracts; this because IC50 values after 24, 48, and 72 h of combined treatments were 40, 20, and 15 μg/mL.
The interaction with bacterial enzymes can also be related to the synergistic mechanism of natural products with antibiotics [ 39], which can be obtained from an extract or from the combination of extracts, synthetic products, antibiotics, and other natural products [ 40, 41].
As stated above, if EA was detected three times in any solvent extract (or combination of extracts) from any resin in either assay, that resin was classified as leaching chemicals having EA and is highlighted in yellow in Figures 2 and 3.
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