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After 24 h, celecoxib, ABL and their combination were added at the selected doses to the culture medium.
Different dilutions of the drugs alone or in combination were added to the cells and incubated for 72 h in a humidified CO2 incubator at 37°C.
After 24 h HGF, PHA-665752 or the combination were added at 40 ng ml−1 or 0.5 μ respectively, and incubated during 72 h.
Pan-caspase inhibitors Z-VAD-Fmk and Q-VD-Oph, used individually or in combination, were added simultaneously with H2O2 or SSP at 100 μmol/L.
Endothelial cell cultures were rendered quiescent for 45 min in serum-free basal medium VEGF-A and PEDF (alone or in combination) were added at 100 ng/ml based on our previous studies (Cai et al, 2006, 2011a).
After adherence of the cells at 37°C and 5% CO2 for 1 hour, histamine (0.1 or 1 mM) or TNF-α (10 or 50 ng/ml) alone or both in combination were added to the serum-free DMEM medium in the upper transwell chamber.
Similar(51)
Then, 10/50/90 (set 1) combination is added to the precedent points.
For the reference, the results of eight system combination without using our proposed combination are added to the last line of 1 ch case [ID 6)].
This precipitate was added along with 10 ml of supernatant solution (magnesium acetate) of 1.0 M concentration and this combination was added gently over the set gels in the test tubes.
Mature cultures were then washed carefully and serum-free medium containing cyclotraxin-B (200 nM) or BDNF (4 nM), alone or in combination, was added to the cells each 24 h for 72 h.
The third component of the combination was added at a single concentration per plate.
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