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This precipitate was added along with 10 ml of supernatant solution (magnesium acetate) of 1.0 M concentration and this combination was added gently over the set gels in the test tubes.
Mature cultures were then washed carefully and serum-free medium containing cyclotraxin-B (200 nM) or BDNF (4 nM), alone or in combination, was added to the cells each 24 h for 72 h.
The third component of the combination was added at a single concentration per plate.
This 362.07 code combination was added for 2006 [ 12]. 2. 362.83 (retinal edema) AND 250.5x (diabetes with ophthalmic manifestations) [ 2].
Either NFV dissolved in DMSO, BZ dissolved in PBS, or the combination was added, and the cells were allowed to grow for an additional 48 or 72 h.
Thereby, inhibition of the three stages, alone or in combination, was added to our experimental design for immortalisation of the mesothelial OSE cells.
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The best combination was adding 1.0, 0.4, and 0.4 mg·L−1·d−1 of trace elements Fe, Co, and Ni (A1B3C2), respectively.
Then, 10/50/90 (set 1) combination is added to the precedent points.
For the reference, the results of eight system combination without using our proposed combination are added to the last line of 1 ch case [ID 6)].
After 24 h, celecoxib, ABL and their combination were added at the selected doses to the culture medium.
Different dilutions of the drugs alone or in combination were added to the cells and incubated for 72 h in a humidified CO2 incubator at 37°C.
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