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The laser pulse is delivered to the skin by the combination probe at the fluence 36 mJ/cm.
The chromosomal localization of the human DRIM locus was clarified and refined by FISH using metaphase spreads obtained from normal human peripheral blood leukocytes, and a combination probe composed of two human DRIM genomic clones.
In order to perform the microdialysis sampling and icv infusion simultaneously, a microdialysis probe with an additional infusion cannula passing through the lumen of probe (IBR combination probe with 1 mm polyacrylanitrile membrane, BASi, West Lafayette, IN, USA) was implanted into the lateral ventricle (coordinates, −0.9 mm anteroposterior, −1.6 mm lateral, −2.9 mm dorsoventral).
Fluorescence in situ hybridization using a panel of seven combination probe sets (Table 1) was attempted on FNA material obtained ex vivo from 122 resected renal cortical neoplasm masses comprising 77 ccRCCs, 19 pRCCs, 14 chrRCCs, 11 OCs and one Xp11-translocation RCC (Table S1).
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After the initial screening of probes, the next step is to select a combination of probe length, probe position and assay stringency that fulfill the criteria for assigning a genotype for each mutation site.
It would seem that if the combination of probe and probe set level normalizations really offered improved performance for a variety datasets, then both types of normalization would be implemented in commonly used algorithms.
Together, combination of probe sets and removal of less reliable probes leads to a new chip definition that is best suited to detect differential splicing of known exons at the expense of the ability to detect novel exons.
One of the most promising strategies in this area is the combination of probe ligation, amplification and microarray detection.
Because for each combination of probe and conditioner, the conditioner level was varied, we have a pair of rate-level functions of the conditioner level.
The horse contigs are ordered and oriented correctly using a combination of probe sequences and positional information from the comprehensive radiation hybrid and FISH maps [ 38].
Of these, 3454 genes were the same as in the original microarray design and represented by the same probes, while 830 genes resulted from merging or splitting of genes between the various genebuilds and were represented by a different combination of probes compared with the original probe combinations.
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