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Truncated variants of Gli1, Gli2, Gli3, as well as Gli2SD1-4 in which Serine-to-Aspartic Acid mutations were introduced at target sites for PKA, were generated by a combination of restriction digestion and PCR strategies.
cl C6 (cl-C6) hRFC was constructed by a combination of restriction digestions and site-directed mutagenesis.
A combination of restriction digestion, PFGE and Southern hybridisation was used to validate the size of pMP1046A.
The classical MspI/HpaII combination of restriction enzymes targeted CpG islands whereas the other methods covered 5mC and 5hmC sites outside of these regions.
We selected this combination of restriction enzymes based on in silico analysis in rice, our experience in sugarcane, and its use in barley.
In opposite, the EcoRI/ MseI recognized cleavage sites on less than half (34%) of the apple full-length cDNA tested, but this combination of restriction enzymes generated more informative TDFs than all the other enzyme pairs tested.
Similar(43)
I feel that stranger danger is reasonably well managed with a combination of restrictions, monitoring and education.
The law encompasses several measures, most of which have been tried in a few other states, though no other state has as broad a combination of restrictions.
China has kept its currency pegged at a fixed exchange rate to the dollar for the past decade, and it has done so by a combination of restrictions on capital flows and heavy purchases of American securities.
The combination of restrictions and time frames — ranging from ten to twenty-five years — is also designed to give the international community more insight into Iran's program and capabilities.
The combination of restrictions on the labor movement, limited democracy, and burgeoning nationalism led many to question the legitimacy of the system.
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